El martes 3 de Septiembre el seminario estará a cargo de María José
Limeres (IMPaM) quien disertará sobre:
"Desarrollo y caracterización de una formulación mejorada de
nanopartículas sólidas lípidas como sistema eficiente en el delivery de
genes"
Los esperamos a las 12:30 hs e el aula del piso 13.
Resumen
The nanoparticle-mediated plasmid delivery is considered a useful tool to
introduce foreign DNA into the cells for the purpose of DNA vaccination
and/or gene therapy. Cationic solid lipid nanoparticles (cSLNs) are
considered one of the most promising non-viral vectors for nucleic acid
delivery. Based on the idea that the optimization of the components is
required to improve transfection efficiency, the present study aimed to
formulate and characterize cholesteryl oleate-containing solid-lipid
nanoparticles (CO-SLNs) incorporating protamine (P) to condense DNA so as
to produce P:DNA:CO-SLNs complexes as non-viral vectors for gene delivery
with reduced cytotoxicity and high cellular uptake efficiency.
