Laboratorio de Patogenia Bacteriana (PatoBac)
Directora:
Dra. Buzzola, Fernanda 
Integrantes:
Dr. Dotto, Cristian
Dr. Sordelli, Daniel
Tec. Medina, Lorena
Pasantes de Investigación:
Sanchez, Candela
Viñuela, Camila
Isolabella Casimiro, Nicolás Tao
Estudiamos aspectos relacionados con la patogenia y el control de las infecciones bacterianas en el hombre y en bovinos. Nos interesa entender los mecanismos adaptativos de Staphylococcus aureus a diferentes nichos evaluando los factores involucrados y la plasticidad genómica. Analizamos los efectos que ejercen ciertos agentes farmacológicos o sus derivados sobre la biopelícula bacteriana. Usamos una variedad de técnicas de la microbiología clásica, molecular y celular, herramientas bioinformáticas y modelos murinos de infección.
Publicaciones destacadas:
- Bonsaglia ECR, Calvo GH, Sordelli DO, Silva NCC, Rall VLM, Casas A, Buzzola F. The Impact of Low-Level Benzalkonium Chloride Exposure on Staphylococcus spp. Strains and Control by Photoinactivation. Antibiotics. 2023; 12(8):1244. https://doi.org/10.3390/antibiotics12081244
- Cáceres ME, Ledesma MM, Lombarte Serrat A, Vay C, Sordelli DO, Giacomodonato MN, Buzzola FR. Growth conditions affect biofilms of Staphylococcus aureus producing mastitis: Contribution of MALDI-TOF-MS to strain characterization. Curr Res Microb Sci. 2021 Oct 5;2:100073. doi: 10.1016/j.crmicr.2021.100073.
- Dotto C, Lombarte Serrat A, Ledesma M, Vay C, Ehling-Schulz M, Sordelli DO, Grunert T, Buzzola F. Salicylic acid stabilizes Staphylococcus aureus biofilm by impairing the agr quorum-sensing system. Sci Rep. 2021 Feb 3;11(1):2953. doi: 10.1038/s41598-021-82308-y.
- Volpe V, Giacomodonato MN, Sordelli DO, Insausti M, Buzzola FR, Grünhut M. Ciprofloxacin loaded o/w microemulsion against Staphylococcus aureus. Analytical and biological studies for topical and intranasal administration. Journal of Drug Delivery Science and Technology, Volume 57, 2020, 101705, doi: 10.1016/j.jddst.2020.101705.
- Bohm GC, Gándara L, Di Venosa G, Mamone L, Buzzola F, Casas A. Photodynamic inactivation mediated by 5-aminolevulinic acid of bacteria in planktonic and biofilm forms. Biochem Pharmacol. 2020 Jul;177:114016. doi: 10.1016/j.bcp.2020.114016.
- Suligoy CM, Díaz RE, Gehrke AK, Ring N, Yebra G, Alves J, Gómez MI, Wendler S, Fitzgerald JR, Tuchscherr L, Löffler B, Sordelli DO, Llana MN, Buzzola FR. Acapsular Staphylococcus aureus with a non-functional agr regains capsule expression after passage through the bloodstream in a bacteremia mouse model. Sci Rep. 2020 Aug 24;10(1):14108. doi: 10.1038/s41598-020-70671-1.
- Marbach H, Mayer K, Vogl C, Lee JYH, Monk IR, Sordelli DO, Buzzola FR, Ehling-Schulz M, Grunert T. Within-host evolution of bovine Staphylococcus aureus selects for a SigB-deficient pathotype characterized by reduced virulence but enhanced proteolytic activity and biofilm formation. Sci Rep. 2019 Sep 17;9(1):13479. doi: 10.1038/s41598-019-49981-6.
- Grunert T, Stessl B, Wolf F, Sordelli DO, Buzzola FR, Ehling-Schulz M. Distinct phenotypic traits of Staphylococcus aureus are associated with persistent, contagious bovine intramammary infections. Sci Rep. 2018 Oct 29;8(1):15968. doi: 10.1038/s41598-018-34371-1.
- Suligoy CM, Lattar SM, Noto Llana M, González CD, Alvarez LP, Robinson DA, Gómez MI, Buzzola FR, Sordelli DO. Mutation of Agr Is Associated with the Adaptation of Staphylococcus aureus to the Host during Chronic Osteomyelitis. Front Cell Infect Microbiol. 2018 Feb 2;8:18. doi: 10.3389/fcimb.2018.00018.
- Dotto C, Lombarte Serrat A, Cattelan N, Barbagelata MS, Yantorno OM, Sordelli DO, Ehling-Schulz M, Grunert T, Buzzola FR. The Active Component of Aspirin, Salicylic Acid, Promotes Staphylococcus aureus Biofilm Formation in a PIA-dependent Manner. Front Microbiol. 2017 Jan 23;8:4. doi: 10.3389/fmicb.2017.00004.
- Gándara L, Mamone L, Bohm GC, Buzzola F, Casas A. Enhancement of photodynamic inactivation of Staphylococcus aureus biofilms by disruptive strategies. Lasers Med Sci. 2017 Nov;32(8):1757-1767. doi: 10.1007/s10103-017-2253-3.
- Grunert T, Wenning M, Barbagelata MS, Fricker M, Sordelli DO, Buzzola FR, Ehling-Schulz M. Rapid and reliable identification of Staphylococcus aureus capsular serotypes by means of artificial neural network-assisted Fourier transform infrared spectroscopy. J Clin Microbiol. 2013 Jul;51(7):2261-6. doi: 10.1128/JCM.00581-13.
- Barbagelata MS, Alvarez L, Gordiola M, Tuchscherr L, von Eiff C, Becker K, Sordelli D, Buzzola F. Auxotrophic mutant of Staphylococcus aureus interferes with nasal colonization by the wild type. Microbes Infect. 2011 Nov;13(12-13):1081-90. doi: 10.1016/j.micinf.2011.06.010.
- Alvarez LP, Barbagelata MS, Cheung AL, Sordelli DO, Buzzola FR. Salicylic acid enhances Staphylococcus aureus extracellular adhesin protein expression. Microbes Infect. 2011 Nov;13(12-13):1073-80. doi: 10.1016/j.micinf.2011.06.003.
- Tuchscherr L, Löffler B, Buzzola FR, Sordelli DO. Staphylococcus aureus adaptation to the host and persistence: role of loss of capsular polysaccharide expression. Future Microbiol. 2010 Dec;5(12):1823-32. doi: 10.2217/fmb.10.147.
- Alvarez LP, Barbagelata MS, Gordiola M, Cheung AL, Sordelli DO, Buzzola FR. Salicylic acid diminishes Staphylococcus aureus capsular polysaccharide type 5 expression. Infect Immun. 2010 Mar;78(3):1339-44. doi: 10.1128/IAI.00245-09.